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Guanidine thiocyanate for use

August 07, 2024
  Guanidine isothiocyanate is white crystalline, melting point 116 ~ 121℃, at 20℃, the PH value of 6mol/L in water is 4.5 ~ 6, and the solubility is completely dissolved. Is a kind of uncoupling agent, is a kind of strong egg Chemicalbook white matter denaturant, can dissolve protein, and make protein secondary structure disappear, cell structure degradation, nuclear protein quickly separated from nucleic acid. For denatured lytic cells; rna and dna were extracted without RNase and DNase activity.
  Guanidine thiocyanate can be used as biochemical reagent and pharmaceutical intermediate. Used for fat industry degreasing, daily chemical industry as a strong lipid deactivator, biopharmaceutical cell membrane rupture agent.
  Research use: Guanidine thiocyanate has been used:
1. The colorectal adenocarcinoma cell line is lysed prior to RNA isolation
2. Used as a PCR inhibitor for triple mitochondrial DNA(mtDNA)qPCR detection of guanidine thiocyanate is a denaturant often used for RNA isolation. It is used as a storage buffer for whole blood samples.
Guanidine thiocyanate inactivates nucleases and is ideal for storing and freezing fecal samples for DNA studies. It is used in combination with phenol-chloroform in RNA extraction.
  Application in the field of chemical hair removal agents:
Chemical hair removal agent is a hair removal agent that can make the hair soft, reduce the strength of the hair, and wipe or rinse the hair in 2 to 6 minutes. Because the composition of the hair shaft is similar to that of the skin, chemical hair removal agents will inevitably damage the skin, so hair removal agents are listed as special use cosmetics.
Thioglycolic acid hair removal agent is the main hair removal agent nowadays, it gradually replaces sulfide hair removal agent. The hair removal active substances used in this kind of hair removal agent are thioglycolic acid and its salt, mercaptopropionic acid and its salt, mercaptolactic acid and its salt. Considering the performance and price, thioglycolic acid and its salts are mainly used as hair removal active substances. The application of some Swellingagents helps to speed up the speed of hair removal by sulfhydryl compound hair removal agents, thereby reducing the irritating effect on the skin. Such swelling agents include melamine, dicyanodiamide or a mixture of both (w=0.5% ~ 2%), thiourea, potassium thiocyanate, guanidine thiocyanate and PVP copolymers.
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